Papain is referred to as papain, also known as papain. It is a natural biological enzyme product extracted by modern bioengineering technology using milk in the immature papaya fruit. It is a sulfhydryl (-SH) endopeptidase with protease and esterase activities, broad specificity, and strong hydrolysis ability for animal and plant proteins, polypeptides, esters, amides, etc., also has the function of synthesis, can synthesize protein hydrolyzate into protein-like.

Chemical properties of papain:

Soluble in water and glycerol, the aqueous solution is colorless or light yellow, sometimes milky white; almost insoluble in organic solvents such as ethanol and chloroform. It is suitable for a pH value of 6~7 (generally 3~9.5), and also works when it is neutral or slightly acidic. The isoelectric point (pI) is 8.75; the suitable temperature is 55~65℃ (generally 10~85℃), It has a strong heat resistance and will not be completely inactivated at 90 °C; it is inhibited by oxidants and activated by reducing substances.

Medical industry application of papain:

1. Food industry applications

Enzymatic reactions can be used to hydrolyze the protein of food macromolecules into small molecular peptides or amino acids. It is used for hydrolyzing animal and vegetable protein, making meat tenderizer powder, hydrolyzing sheep placenta, hydrolyzing soybean, loosening agent for biscuits, noodle stabilizer, clarifying agent for beer beverage, advanced oral liquid, health food, soy sauce brewing, and alcoholic starter, etc. Effectively converting the utilization of protein, greatly improve the nutritional value of food and reduce costs. Conducive to human digestion and absorption.

2. Cell Culture

Papain is used to isolate cells in the first step of preparing the cell culture medium. After 10 minutes of the enzyme treatment of small tissue pieces, the extracellular matrix connecting cells can be interrupted; then protease inhibitors are used to stop the reaction to prevent papain from further lysing the cells themselves; finally, the tissue pieces are broken up with Pasteur pipette. single cell suspension.

3. Immunology

In immunology, papain can be used to cleave apart the Fc fragment (which can be crystallized) and the Fab fragment (antigen binding) of immunoglobulins (ie antibodies).

Our plantextractstory company can provide you with papain powder-free samples for the majority of users, if you have this demand, welcome to consult!